SubtiBank SubtiBank
ytkP [2019-08-08 13:59:14]
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ytkP [2019-08-08 13:59:14]

similar to cysteine synthase
locus
BSU_29970
pI
4.93
mw
32.91 kDa
protein length
311 aa Sequence Blast
gene length
933 bp Sequence Blast
function
unknown
product
unknown
essential
no
ec
2.5.1.47
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    3,066,451 → 3,067,386

    The protein

    Catalyzed reaction/ biological activity

  • O(3)-acetyl-L-serine + H2S = L-cysteine + acetate (according to UniProt)

    • Protein family

  • Cysteine synthase/cystathionine beta-synthase family (with [protein|D1FC976597E5583E40A4ED7234FBCA743AB01354|CysK] and [protein|F195C47849F4F6B4C56E3A386F5D8C84326B01C4|MccA], according to UniProt)

    • Paralogous protein(s)

  • [protein|D1FC976597E5583E40A4ED7234FBCA743AB01354|CysK], [protein|F195C47849F4F6B4C56E3A386F5D8C84326B01C4|MccA]

    • [SW|Cofactors]

  • PLP (according to UniProt)

    • Structure

  • [PDB|2EGU] (from Geobacillus Kaustophilus 57% identity)

    • [SW|Localization]

  • cytoplasm (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|6F1462EA074DEDE2FEF13561A6691A22BFAD10FE|ytkP]
    description
    [Pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • MGNA-B534 (ytkP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1533 NBRP B. subtilis, Japan]
  • BKE29970 (Δ[gene|6F1462EA074DEDE2FEF13561A6691A22BFAD10FE|ytkP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTCATTCCTTTCTGAA, downstream forward: _UP4_TAAAAAAAGGACTGGCTTCA
  • BKK29970 (Δ[gene|6F1462EA074DEDE2FEF13561A6691A22BFAD10FE|ytkP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTCATTCCTTTCTGAA, downstream forward: _UP4_TAAAAAAAGGACTGGCTTCA